JSONPATH-transformation failed

Unless I really misunderstand JSON, you can’t extract the temperature based on the ID attribute - they are not related other than being at the same level. You can visualise the levels like so:

{
	"Time": "2020-06-20T13:35:52",
	"DS18B20_1": {
		"Id": "3C01A8160EFB",
		"Temperature": 34.4
	},
	"DS18B20_2": {
		"Id": "3C01A8162221",
		"Temperature": 28.3
	},
	"DS18B20_3": {
		"Id": "51D7FD1D64FF",
		"Temperature": 35.5
	},
	"TempUnit": "C"
}

As you can see, DS18B20_1 is the ‘name’ of a group of attributes, which are Id and Temperature. Using transformationPattern to extract temperature, you need:

$.DS18B20_1.Temperature

To be clear, you can’t extract Temperature using Id.

EDIT:
To perhaps give you more to work with, see my thing below. This is a Sonoff Basic switch with a DHT22 sensor attached, flashed with Tasmota. My MQTT broker is Mosquitto, which I have defined in a separate things file and is referenced in the (mqtt:broker:MosquittoMqttBroker) part of the thing definition.

    // Switch Bedroom Desk Light
	Thing mqtt:topic:swBedroomDeskLight "Switch Bedroom Desk Light" (mqtt:broker:MosquittoMqttBroker) {
		Channels:
			Type switch : switch "Power Switch" [ 
				stateTopic="stat/swBedroomDeskLight/POWER", 
				commandTopic="cmnd/swBedroomDeskLight/POWER",
				on="ON",
				off="OFF"          
			]
			Type switch : reachable "Reachable" [
				stateTopic = "tele/swBedroomDeskLight/LWT",
				on="Online",
				off="Offline"
			]
			Type number : temperature "Temperature" [ 
				stateTopic="tele/swBedroomDeskLight/SENSOR",
				transformationPattern="JSONPATH:$.AM2301.Temperature"
			]
			Type number : humidity "Humidity" [ 
				stateTopic="tele/swBedroomDeskLight/SENSOR",
				transformationPattern="JSONPATH:$.AM2301.Humidity"
			]
			Type string : tempunit "TempUnit" [ 
				stateTopic="tele/swBedroomDeskLight/SENSOR",
				transformationPattern="JSONPATH:$.TempUnit"
			]
			Type switch:state "State" [
				stateTopic="tele/swBedroomDeskLight/STATE",
				transformationPattern="JSONPATH:$.POWER",
				on="ON",
				off="OFF"
			]
	}

The JSON string received on tele/swBedroomDeskLight/SENSOR looks very similar to yours:

{"Time":"2020-06-20T07:43:44","AM2301":{"Temperature":21.1,"Humidity":65.1},"TempUnit":"C"}

Because Temperature is within the group AM2301, my transformationPattern is:

JSONPATH:$.AM2301.Temperature